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615.9+577.472(28):614+577.4 SALVAGE NAD .. , .. , .. , .. - ...

66 . , 2013, 1(4)

615.9+577.472(28):614+577.4

SALVAGE NAD

.. , .. , .. , ..

- ,

670013, . -, . , 40-, vtsyrenov@gmail.com.

, NAD NADP (.. 2.7.4.3), salvage NAD. salvage .

. 9. . 1. . 4 .

: NAD; ; ; .

ROLE OF ADENYLATE KINASE IN SALVAGE SYNTHESIS OF NAD BY

CORYNEFORM BACTERIA

V.Zh. Tsyrenov, L.V. Tulokhanova, E.M. Podlepa, A.A. Sandanov East Siberian State University of Technology and Management, 40v Kluchevskaya st., Ulan-Ude, 670013, Russia, vtsyrenov@gmail.ru.

Inhibition action of NAD and NADP during biosynthesis of them by coryneform bacteria was shown to be attracted to adenylete kinase (E.C. 2.7.4.3), which is member of adenylate branch of NAD biosynthesis salvage pathway. Pattially purified adenylatekinase was also inhibited by intermediate metabolytes of salvage synthesis of NAD.



9 figures. 1 table. 4 sources.

Keywords: NAD; adenylatekinase; ATP; Biosynthesis.

300 - NAD NADP [1]. - , . , .

. , 2013, 1(4) salvage [2]. - (Davis, 1964) 10% () , - ().

. .

NAD - 100% NAD - . . , NAD , , , .

: Corynebacterium ammoniagenes 6872, Corynebacterium fla- NAD, NADP, NADH NAD vum 301, Corynebacterium ammoniagenes , 403 24 : NAD .. . .





- , Sal- NAD, NADP NADH , vage [2]. NAD. - NAD - 4 /. NADP (/): 20; 10; (.1).

- - , salvage () 10; NaC1 3; 30 ; NAD . 7,37,4. - , - (/): 100; 6: 2O4 NAD NADP , 2O4 10; 10; 12 0,1; MgSO4 H20 10; 30 ; - NAD.

7,37,4; , ; AMP 0,5 . 30 . . NAD NADP - AMP , 660 - AMP -26 [3] NAD (3 /) HADP (2 /). NAD NAD NADP - 56%, NADP 17%. [1]. AMP, - , Whatman - NAD NADP NAD : AMP.

(7:3), Silufol UV 254 - : - AMP : 1 N - [ ] AMP.

- -HCl ( 7,5) - AMP 20000 g 20 . 5 (. 2).

20x180 DEAE- , TOYOPEARL HW-65.

[ ]- C. ammoniagenes 6872 1 N 1 ADP . 3. c Inter- technique (). 35 55% .

, 1 . [4]. 50% NAD NADP 68 . , 2013, 1(4)

   

3 3,5 . (. 3). , NAD NADP

   

. 3 NAD (1) NADP (2)

. , 2013, 1(4)

   

. 8. FMH (1) FAD (2) ; FAD ( ) ; FAD

72 . , 2013, 1(4)

   



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